The Saint Agnes Chamber Choir, a group dedicated to singing the masterpieces of the High Renaissance, performed for the first time in 1986. Jenny Geach organized a group of women to join the Schola Cantorum and sing William Byrd's Mass for Three Voices. Mary Le Voir directed the newly formed group. The Chamber Choir continued to sing on a regular basis but not on a set schedule. In 1991 Donna May became the director of the Choir, and in 1993 the Chamber Choir began to sing the anticipatory Mass of the 1st Sunday of each month during the regular Twin Cities Catholic Chorale season (Oct. - June). The Choir's repertoire includes more than a dozen Renaissance Masses and scores of polyphonic motets, both of Renaissance and contemporary composers.

The Chamber Choir is listed on Brad Leissa's List of Early Music Vocal Ensemble.